MARINE OXIDATION FERMENTATION MEDIUM (MOF MEDIUM) (VEG.)

Description

This medium is prepared by replacing Casein enzymic hydrolysate with Veg hydrolysate which is free from BSE/ TSE risks. MOF Veg Medium is the modification of MOF Medium which is a modified version of the formula originally developed by Leifson; used for differentiating oxidative and fermentative carbohydrate metabolising marine bacteria. For differentiating the fermentation and oxidation of carbohydrates, inoculate two tubes of medium containing carbohydrate with each culture to be tested. Cover one tube of each culture by sterile melted petrolatum to form a layer about one inch in height. The marine bacteria which change the colour of the medium in both the tubes from red to yellow are carbohydrate fermenters and those which change the medium from red to yellow in the open (uncovered) tube only, are carbohydrate oxidizers. No change in the covered medium and an alkaline reaction in the uncovered medium by the marine bacteria are neither oxidative nor fermentative.