MegaFiTM Pro One-Step RT-PCR

Description

MegaFi Pro One-Step RT-PCR is a highly efficient, all-in-one solution for performing both reverse transcription and PCR amplification in a single reaction. The kit includes two essential components: the RT-PCR enzyme mix, which combines OneScript Hot Reverse Transcriptase Cat. No. G593 , MegaFi Pro Fidelity DNA Polymerase Cat. No. G886 , and RNaseOFF Ribonuclease Inhibitor Cat. No. G138 , and the 2X One-Step RT-PCR Buffer, which contains gel loading dye and all other necessary reagents. The enzyme mix ensures exceptional sensitivity and high fidelity with over 2000X lower error rates, while the buffer is optimized with stabilizers and enhancers provides flexiblity in choosing desired primers to support robust reactions across a variety of RNA templates. This streamlined system simplifies the process, offering a reliable, one-step alternative to traditional two-step RT-PCR protocols. Product Features: Enjoy simple, one-step RT-PCR reactions Extremely thermostable cDNA synthesis at 50-80C Excellent protection against common ribonucleases Over 2000X lower error rates Included in abms PCR Buffet Program Product Component Quantity RT-PCR Enzyme Mix 100 rxn 400 mul 2X One-Step RT-PCR Buffer 2 x 1.25 ml ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization .