MEM alpha, No Nucleosides Medium

Description

MEM alpha, or Minimum Essential Medium alpha, is modified to contain Earles salts, and is without nucleosides. This basal medium is used with a variety of suspension and adherent mammalian cells, and as a selection for transfected DHFR-negative cells. Supplementation may be required, most commonly10 % fetal bovine serum FBS for cell culture use, and requires a 5-10 % CO2 environment to maintain physiological pH. MEM alpha is modified as follows: With L-Glutamine Earles Salts 2.2 g/L Sodium Bicarbonate Phenol Red Without Ribonucleosides Deoxyribonucleosides Special media modification is available as a Custom Media Service, please contact a technical representative at technical@abmgood.com for more details.