MightyPrep Reagent for DNA

Description

MightyPrep reagent for DNA is designed to easily prepare DNA extracts that can be used directly as PCR templates-from animal tissues such as mouse tail , plant tissues, blood, processed food, and soil. DNA extracts are prepared using a simple protocol that consists of adding the reagent to a sample, then incubating and centrifuging the mixture. These extracts can be used directly as PCR templates with inhibitor-resistant PCR enzymes such as MightyAmp DNA Polymerase Ver.3 Cat. #R076A/B or Tks Gflex DNA Polymerase Cat. #R060A/B to successfully amplify targets. This product can also be used to efficiently prepare DNA extracts from microorganisms. The resulting extracts can be used as templates with the Bacterial 16S rDNA PCR Kit Fast 800 Cat. #RR182A or the Fungal rDNA ITS1 PCR Kit Fast Cat. #RR183A .: N/A in all geographical locations. Check for availability in your region. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Amplification of DNA extracted from various microorganisms using MightyPrep Reagent for DNA. DNA extracts were prepared from colonies of various microorganisms five bacterial and three fungal species using MightyPrep reagent for DNA. These bacterial and fungal extracts were then used as templates with PCR kits specially designed for amplifying the bacterial 16S ribosomal DNA rDNA region and the fungal ribosomal DNA rDNA ITS1 region, respectively. All of the DNA extracts tested were successfully amplified. Lane M1: 250 bp DNA ladder; Lane 1:Salmonella entericavarEnteritidis;Lane 2:Bacillus subtilis;Lane 3:Enterococcus faecalis; Lane 4:Staphylococcus aureus; Lane 5:Escherichia coli; Lane M2: 100 bp DNA ladder; Lane 6:Candida boidinii; Lane 7:Candida tropicalis; Lane 8:Saccharomyces cerevisiae. . Back