MINERAL MODIFIED GLUTAMATE MEDIUM BASE (DOUBLE STRENGTH)

Description

Mineral Modified Glutamate Medium is used for enumeration of coliforms in water and wastewater samples. Folpmers first described a chemically defined medium based on glutamic acid for enumerating coliforms in water. However, in the early days following its discovery, it was seen that the medium containing glucose gave many false positive results in 48 hours. It was then modified by Gray who incorporated formate and lactose in the medium which gave improved performance. This medium can also be used for detecting Escherichia coli in chlorinated waters and is better than Lauryl Tryptose Lactose Broth for the detection of small numbers of Escherichia coli. Presumptive positive tubes must be subcultured into Lauryl Tryptose Mannitol Broth and Brilliant Green Bile Broth and incubated at 44?C to detect indole formation at this temperature before identifying Escherichia coli.