Mir-XTM miRNA First-Strand Synthesis Kit

Description

This 20 rxn kit contains reagents for synthesizing first-strand cDNA from either total RNA or purified small RNAs isolated from any source. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly A polymerase and SMART MMLV RT. The resulting cDNA and Takara Bios TB Green Advantage qPCR Premix can be used in a real-time qPCR analysis to quantify specific miRNAs and other RNAs present in the original sample. For 60 rxn kit see Cat. # 638315. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry.In the Mir-X cDNA synthesis reaction, RNAs are poly A -tailed using poly A polymerase, and then copied using a modified oligo dT primer and SMART MMLV Reverse Transcriptase. Back Specific quantification of Let7 miRNA variants.Using miRNA-specific primers Panel A , Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly A +RNA Panel B .The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations. Back Back Back