miRNA All-In-One cDNA Synthesis Kit

Description

The miRNA All-In-One cDNA Synthesis Kit provides a comprehensive solution for efficient synthesis of first-strand miRNA cDNA from RNA samples. The kit includes all necessary components for a hassle-free setup, enabling RNA molecules to undergo polyadenylation and reverse transcription in a simple, single-tube reaction. This process uses Poly A Polymerase, OneScript Plus Reverse Transcriptase, and a modified Oligo d T with Adapter that anneals to poly A -tailed miRNA. The included SuperMix contains RNaseOFF Ribonuclease Inhibitor to protect RNA from degradation. The synthesized first-strand miRNA cDNA can be directly used as a template for qPCR-based miRNA expression analysis with BlasTaq qPCR MasterMix sold separately, Cat. No. G891 . Users can choose from abms extensive collection of miRNA qPCR primers, covering all known miRNA sequences. Each primer set includes a unique miRNA Forward Primer and a Universal 3 miRNA Reverse Primer that binds to the complementary Adapter sequence on the modified Oligo d T . Product Component Quantity 2X miRNA cDNA Synthesis SuperMix 20 rxn 200 mul Enzyme Mix 20 rxn 40 mul Nuclease-Free H2O 1.0 ml