Mouse bACE1 (Beta-Site APP Cleaving Enzyme 1) ELISA Kit

Description

Synonym: ASP2; Beta Secretase; Memapsin 2; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Membrane-associated aspartic protease 2. This Mouse bACE1 (Beta-Site APP Cleaving Enzyme 1) ELISA Kit applies to the in vitro quantitative determination of Mouse bACE1 concentrations in serum, plasma and other biological fluids. Type:Sandwich, Assay time:3.25h, Detection type:Colormetric, Precision:CV<10%, Assay Range:0.15-10ng/mL, Sensitivity:0.07ng/mL, Recovery:80%-120%, Cross reactivity:The kit detected Mouse bACE1 in the samples and no significant cross-species reactions were found. Detection Principle:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse bACE1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse bACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse bACE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse bACE1. You can calculate the concentration of Mouse bACE1 in the samples by comparing the OD of the samples to the standard curve.