Mouse β-EP (Beta-Endorphin) ELISA Kit

Description

Synonym: b-EP. This Mouse β-EP (Beta-Endorphin) ELISA Kit applies to the in vitro quantitative determination of Mouse β-EP concentrations in serum, plasma and other biological fluids. Type:Competitive, Assay time:1.5h, Detection type:Colormetric, Precision:CV<10%, Assay Range:15.63-1000pg/mL, Sensitivity:9.38pg/mL, Recovery:80%-120%, Cross reactivity:The kit detected Mouse β-EP in the samples and no significant cross-species reactions were found. Detection Principle:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse β-EP. During the reaction, Mouse β-EP in the sample or standard competes with a fixed amount of Mouse β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.