Mouse Oral Carcinoma Cell Line (MOC-L1)

Description

Oral squamous cell carcinoma OSCC was induced in mice using 4NQO. This cell line shows green fluoresence and expresses a range of epithelial markers. MOC-L1 carries a missense mutation in the DNA binding domains of p53 gene. Furthermore, the cell line displays high levels of epithelial-mesenchymal transition and associated characteristics. These cells are clonogenic, in vitro, and have tumorigenic properties. MOC-L1 exhibits potential for local regional and distal metastasis. Western blot, qPCR and IHC were used for epithelium expression, GFP tag, EMT and stemness markers, as well as to confirm the murine nature of the cells. As these cell lines are derived from C57BL/6 mice, they are useful for studies investigating OSCC cells with other genetically modified cells, as well as, OSCC pathogenesis.