MRC5 2G HCP ELISA Kit

Description

The MRC5 2G HCP ELISA Kit is a simple-to-use, objective, and semi-quantitative ELISA kit intended for use in determining the presence of host cell protein impurities in products manufactured by expression in MRC5 cells. The antibodies used in this 2nd generation MRC5 HCP ELISA Kit, F1090, have been raised against and affinity purified using HCP recovered from MRC5 cell lysate. The antibodies were characterized by Antibody Affinity Extraction AAE and Mass Spectrometry, demonstrating coverage to majority of HCPs in MRC5 cells. This kit has LOD 1.8 ng/ml and LLOQ 10ng/ml. It was qualified for testing of final product HCPs by using actual in-process and final drug substance samples. Each user of this kit is encouraged to perform a similar qualification study to demonstrate it meets their analytical needs. Please use Sample Diluent, Item No. I700, with these kits; available separately. This kit is replacing Cygnus MRC5 HCP ELISA Kit, Item # F300.