MUCATE BROTH

Description

Mucate Broth is prepared based on the formula originally developed by Kauffman and Petersen and recommended by APHA for identification of enteropathogenic Escherichia coli from milk and milk products. This medium can also be used as an aid in differentiation of Enterobacteriaceae especially within Salmonella genus. Transfer a loopful of 24 hour Tryptone Broth culture to Mucate Broth. Include Mucate Control Broth tube as a control because occasionally un-inoculated tubes of Mucate Broth turn blue on standing. Incubate at 48 ? 1 hour at 35?C. A negative test result is indicated by a blue or unchanged colour in this broth. 90 % of the E. coli strains are mucate positive.