MUG LAURYL SULPHATE BROTH

Description

Lauryl Sulphate Broth was formulated by Mallmann and Darby and is recommended by APHA for the detection and enumeration of coliform organisms in foods, water and wastewater. MUG is added in Lauryl Sulphate Broth as the fluorogenic compound which permits the rapid detection of Escherichia coli when observed under UV light where further confirmation is not required. MUG detects anaerogenic strains which may not be detected in the conventional procedure. Feng and Hartman used MUG-containing medium for studying ?-glucuronidase activity and found Escherichia coli has 96-100% activity, Salmonella species with 17% and Shigella species 40% activity and other genera were negative. For weakly positive strains incubation should be carried out overnight. Robison reported no false negative results and about 5% false positive results.