Mycoplasma qPCR Detection Kit

Description

Routine Monitoring Made Simple. Screen your cell cultures regularly with the fastest, most reliable qPCR kit. Inhibitor-Resistant MasterMix. Engineered to minimize inhibition and false negatives for confident results. Ultra-Sensitive Detection. Detects as few as 10 copies with complete elimination of NTC signals. abms Mycoplasma qPCR Detection Kit, branded as the #1 qPCR Mycoplasma Detection Kit, is the most advanced solution in our trusted Mycoplasma Detection product line, designed specifically for routine monitoring of cell cultures. This ultra-sensitive, qPCR-based kit enables rapid detection of both Mycoplasma and Acholeplasma directly from culture supernatantno DNA extraction, sample preparation, or gel electrophoresis required. Powered by our inhibitor-resistant 2X MegaFi Pro qPCR MasterMix, the kit effectively minimizes qPCR inhibition and reduces the risk of false negatives, ensuring maximum confidence in your results. With the ability to detect as few as 10 copies and complete elimination of no-template control NTC signals, you can trust this kit to safeguard even your most sensitive workflows. Delivering results in under 2 hours, the kit is the ideal quality control tool for routine screening and contamination prevention in research and production labs. Each kit comes with a separate ROX Reference Dye, providing broad compatibility with a wide range of qPCR instruments. For details, see our ROX Machine Compatibility Chart. Kit Features: #1 qPCR Detection Kit: Our most advanced Mycoplasma testing solution Routine Monitoring: Optimized for regular cell culture contamination screening Inhibitor-Resistant MasterMix: Minimizes qPCR inhibition and reduces false negatives Direct Use: Add diluted mediano prep or extraction needed Fast & Sensitive: Results in under 2 hours; detects as few as 10 copies Highly Specific: Eliminates non-specific and NTC signals Ready-to-Use: Includes all required reagents, including ROX dye qPCR Compatible: Broadly compatible with real-time PCR systems Part of abms trusted Mycoplasma Control product line Product Components Quantity MegaFi Pro 2X qPCR MasterMix 1.25 ml Primer Mix 100 mul Positive Control 50 mul ROX Reference Dye 15 mul Nuclease-Free H2O 2 x 1.0 ml Save when you buy abms Mycoplasma qPCR Detection Kit bundled with MycoAway 1000X Mycoplasma Cocktail Cat. No. G398 . Click here and add this bundle to your cart to save instantly ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization . Why test your cells for Mycoplasma? Due to its small size, Mycoplasma can be present in your growth media without being visibly detected. This can cause significant errors in your experiments due to its effects on: proliferation metabolism gene synthesis & processing adhesion properties and more To safeguard your research, routine monitoring is essential. abms Mycoplasma qPCR Detection Kit provides a fast, ultra-sensitive, and reliable solution for regular screening of your cell cultures, helping to maintain data integrity and experimental reproducibility. Many publishers now request Mycoplasma testing to be reported along with manuscript submissions to ensure accuracy in data reporting. Visit our learning resources center to read more about Mycoplasma contamination. Note: Mycoplasma qPCR Detection Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below. MACHINE TYPE MACHINE High ROX Machines ABI 7000, 7300, 7700, 7900, 7900HT, StepOnePlus, StepOne, OpenArray, PRISM Sequencing Detection Series Biometra TOptical Fluidigm BioMark Wafergene SmartChip System TianLong TL998 System Low ROX Machines ABI 7500, 7500 Fast, Viia 7, QuantStudio, QuantStudio 3/5/6/7 BioGene InSyte Illumina Eco Analytikjena qTower Series Stratagene Mx3000, Mx3005, Mx4000 No ROX machines BioRad CFX96, CFX384, Chromo4, CFX Connect, Opticon 2, MiniOpticon Roche LightCycler 480, 1536, Nano MJ Research Opticon, Opticon 2, Chromo 4 Eppendorf Realplex 4 BioGene SynChron Corbett Rotor-gene 3000, 6200, 62H0, 6500, 65H0, 6600 Eppendorf Mastercycler realplex s, 4 , 4s , Pro S, 384 , Nexus gradient, eco, flat Cepheid SmartCyler, GeneXpert Enigma ML Idaho LightScanner 24, 32 , RapidCycler2, R.A.P.I.D LT, LT Food , RAZOR EX, JBAIDS Qiagen Rotor-Gene Q, 6000 Takara Dice Thermo Scientific PikoReal DNA-Technology DT96, DTlite, DT-322 Bioer LineGene 3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680 Bioneer Exicycler