N-Decanoyl p-Nitroaniline

Description

N-Decanoyl p-nitroaniline DepNA is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase FAAH activity.1 FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its endogenous substrate anandamide AEA . It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. Exposure of DepNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline ?=13,500 at 410 nm . This allows the fast and convenient measurement of FAAH activity using a 96 well plate spectrophotometer.