NA/NE (Noradrenaline/Norepinephrine) ELISA Kit

Description

Synonym: Norepinephrine;NE;noradrenaline;NA;NAd;norad;4;5-β-trihydroxyphenethylamine;. This NA/NE (Noradrenaline/Norepinephrine) ELISA Kit applies to the in vitro quantitative determination of Universal NA/NE concentrations in serum, plasma and other biological fluids. Type:Competitive, Assay time:1.5h, Detection type:Colormetric, Precision:CV<10%, Assay Range:0.31-20ng/mL, Sensitivity:0.19ng/mL, Recovery:80%-120%, Cross reactivity:The kit detected NA/NE in the samples and no significant cross-species reactions were found. Detection Principle:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NA/NE. During the reaction, NA/NE in the sample or standard competes with a fixed amount of NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NA/NE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NA/NE in tested samples can be calculated by comparing the OD of the samples to the standard curve.