NucleoBond RNA Soil

Description

The NucleoBond RNA Soil kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate high sample inputs in order to yield 110 mug of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples. NucleoBond RNA Soil enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 20 samples. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back NucleoBond RNA Soil procedure. Back High RNA yields with NucleoBond RNA Soil.Various soil samples clay, shore soil, and forest soil were purified in duplicate with NucleoBond RNA Soil using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit Q and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 mul and yields were determined photometrically left panel . In addition, 15 mul of each eluate was analyzed by gel electrophoresis 1 % TAE; right panel . For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit. Back Amplifiable RNA for perfect results with NucleoBond RNA Soil.Various soil samples clay, shore soil, and forest soil were purified in duplicate using NucleoBond RNA Soil and competitor kit Q by following the standard protocol for each kit. RT-PCR was then performed using 40 mul of eluate from each sample amplicon size = 466 bp . Samples prepared using NucleoBond RNA Soil demonstrated lower Cp values relative to corresponding samples prepared using kit Q. Back NucleoBond RNA Soil enables efficient parallel DNA isolation.Various soil samples clay, shore soil, and forest soil were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit Q and the corresponding protocol. DNA samples were eluted in a volume of 100 mul and yields were determined photometrically left panel . In addition, 15 mul of each eluate was analyzed by gel electrophoresis 1 % TAE; right panel . For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.