NucleoBond Xtra BAC

Description

NucleoBond Xtra BAC 25 25 preps for the isolation of low-copy larg construct plasmid DNA - NucleoBond Xtra BAC Columns, buffers, RNase A Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back NucleoBond Xtra BAC is suitable for restriction digestion: BAC DNA 300 kb was isolated in triplicate from 500 mlE. coliDH5a using NucleoBond Xtra BAC and competitor products Q and I .After precipitation, BAC DNA was reconstituted in 1,000 mul TE buffer and 5 mul of each sample was used for analysis on a 1 % TAE agarose gel. Obtained yields: MN: 150 mug, Q: 44 mug, I: 75 mug. Restriction digestion of BAC DNA isolated with NucleoBond Xtra BAC. BAC DNA samples were purified from overnight cultures ofE. coliDH5a transformed with a pBAC10 clone. Approximately 3 mug of DNA from each sample was digested with 3 units of MspI, HindIII, or EcoRI at 37C for 2 hr. Digestions were analyzed on a 1 % TAE agarose gel. Lane 1: undigested BAC DNA, lane 2: MspI-digested, lane 3: HindIII-digested, lane 4: EcoRI-digested, lane M: marker. Back High selectivity and specific characteristics of the optimal silica matrix make this anion exchanger ideal for the purification of very large constructs, such as P1, BACs, and PACs.Using the NucleoBond Xtra BAC kit, it takes just 75 min to isolate, for example, BAC DNA free from any kind of RNA, proteins, metabolites, dyes, and carbohydrates. Low amounts of impurities, which often act as strong inhibitors for enzymatic processing, transfections, transcriptions, etc., are completely removed. Back Back