NucleoMag DNA Bacteria

Description

The NucleoMag DNA Bacteria kit employs scalable magnetic bead technology to enable efficient, high-throughput DNA purification from microbial cultures consisting of Gram-positive or Gram-negative bacteria or fungi such as yeast. The kit workflow can be modified with the addition of a sample homogenization step employing MN Bead Tubes or MN 96 Bead Plates to provide improved sample lysis, and the protocol can be easily automated on common liquid handling platforms. NucleoMag DNA Bacteria utilizes a powerful yet environmentally friendly buffer chemistry that is free of chaotropic salts or hazardous components. Typical downstream applications for DNA purified with the kit include PCR, qPCR, and NGS. Catalog # 744310.1 includes sufficient quantities of reagents and materials for processing 96 samples at the volumes indicated in the kit protocol. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back NucleoMag DNA Bacteria procedure.For optimal DNA yields, complete disruption of sample material is necessary, and can be performed using MN Bead Tubes and/or MN 96 Bead Plates. After sample disruption, Binding Buffer IMB and NucleoMag B-Beads are added to the transferred lysate. Following magnetic separation, NucleoMag B-Beads are washed to remove contaminants and salts using Wash Buffer IMW and 80 % ethanol, respectively. After air drying the NucleoMag B-Beads for 10 min at room temperature, the DNA is finally eluted with Elution Buffer IME. The NucleoMag DNA Bacteria kit can be used either for manual processing or it can be automated on standard liquid handling instruments and magnetic separators. Back More efficient extraction of microbial DNA than competitor kits.DNA was isolated from Gram-positive and Gram-negative bacteria as well as yeast using the NucleoMag DNA Bacteria kit MN, blue bars in comparison with Competitor Q gray bars inPanel Aor Competitor T gray bars inPanel Baccording to the manufacturers recommendations. The isolated DNA was then analyzed by qPCR analysis of 16s and 18s rRNA sequences using the Maxima SYBR Green kit by Thermo Fisher Scientific on an Applied Biosystems 7500 Real-Time PCR System. Resulting CTvalues were consistently lower for DNA isolated using NucleoMag DNA Bacteria than CTvalues obtained with DNA isolated using the competitor kits, suggesting that NucleoMag DNA Bacteria provided more efficient extraction of genomic DNA from the various samples. Back Efficient DNA extraction using either bead-plate or single-tube homogenization.DNA was isolated from various Gram-positive and Gram-negative bacteria using the NucleoMag DNA Bacteria kit in combination with different bead formats for sample homogenization and resulting DNA yields were measured by UV spectrometry. The samples were homogenized by using either racks of prefilled tube strips MN 96 Bead Plates or single bead tubes MN Bead Tubes . The resulting yields for samples homogenized with MN 96 Bead Plates dark blue bars were comparable to the yields obtained via homogenization in the MN Bead Tubes light blue bars . Back Reliable DNA integrity with bead-plate or bead-tube homogenization.DNA was isolated from Gram-positive and Gram-negative bacteria using the NucleoMag DNA Bacteria kit, and samples were homogenized in parallel by using either a rack of prefilled tube strips BP = MN 96 Bead Plate or single bead tubes BT = MN Bead Tube . Both homogenization approaches enabled reliable DNA extraction when used in tandem with the NucleoMag DNA Bacteria kit.