NucleoMag DNA Swab

Description

The NucleoMag DNA Swab kit is designed for high-throughput purification of genomic DNA from various swab specimens, enabling efficient sample processing for a wide array of genetic testing applications. The kit has been validated with both cotton and synthetic swabs e.g., flocked swabs , and employs a magnetic bead-based purification technology that can be readily automated on common liquid handling instruments e.g., Hamilton, Tecan, and Eppendorf platforms or magnetic separators such as the KingFisher Duo or Flex. Following reversible adsorption of nucleic acids to superparamagnetic beads, highly pure DNA is eluted under low-salt conditions and can be incorporated directly in downstream applications such as qPCR or NGS. The NucleoMag DNA Swab kit typically yields 13 mug of DNA per prep, and enables processing of 96 samples in 30 min on the KingFisher Flex excluding sample lysis . Cat. # 744601.1 includes sufficient quantities of reagents and materials for processing 1 x 96 samples. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Assessment of yield and qPCR performance of human genomic DNA isolated from different swab types using NucleoMag DNA Swab.Human genomic DNA was purified from buccal swab specimens obtained from >6 individuals using either cotton or synthetic swabs COPAN FLOQSwabs , respectively. Lysates were prepared using NucleoSpin Forensic Filters and DNA was isolated using automated processing on a KingFisher Flex platform according to the standard protocol for the NucleoMag DNA Swab kit. qPCR performance was evaluated using the Quantifiler Human DNA Quantification assay. Back Sensitive detection of bacterial DNA in human swab specimens using NucleoMag DNA Swab.DNA was isolated from mouth swabs on a KingFisher Flex platform using the standard protocol for the NucleoMag DNA Swab kit. For each sample processed, qPCR analysis targeting the bacterial 16S rRNA gene demonstrated sensitive detection of bacteria.