NucleoMag Pathogen

Description

The NucleoMag Pathogen kit is designed for the isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes. This kit provides reagents and magnetic beads for isolation of 4 x 96 samples. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back The NucleoMag SEP magnetic separator is designed for high-throughput nucleic acid purification. Back Sensitivity screening for pathogen detection in human feces samples. Dilution series of 10-310-4of feces samples were prepared for sensitivity screening using three replicates. Nucleic acid extraction was performed with the NucleoMag Pathogen kit and the competitor kit R. PCR analysis was performed using the RIDA GENE Viral Stool Panel I R-Biopharm and RealStar Clostridium difficile PCR Kit 1.0 Altona Diagnostics . Back Back Back Viral RNA from 19 confirmed SARS-CoV-2-positive swab samples was extracted in duplicate using NucleoMag Pathogen and a competitor kit Q . Viral RNA was quantified using a SARS-CoV-2-specific qRT-PCR assay qScript XLT One-Step RT-qPCR ToughMix + nCoV IP4 assay; Institute Pasteur, Paris . For each sample tested, the resulting CT values indicated that the NucleoMag Pathogen kit performed as well as 3/19 samples , or better than the competitor kit 16/19 samples . For two samples tested, detection of SARS-CoV-2 RNA was possible with NucleoMag Pathogen but not with the competitor kit. Back Dilution series were prepared for three different sample types nasal swab, oral swab, saliva using inactivated SARS-CoV-2 virus. Viral RNA was extracted using NucleoMag Pathogen on a KingFisher Flex system and the RNA was quantified by qRT-PCR AgPath-ID One-Step RT-PCR mix + nCoV IP4 assay, Institute Pasteur, Paris . For each sample type, viral RNA was detected consistently and reliably over a 5-log range of dilutions. Back Viral RNA was extracted from 200-mul inputs of diluted SARS-CoV-2-positive saliva samples in duplicate using NucleoMag Pathogen MN and the MagMAX Viral RNA Isolation Kit T , respectively, and quantified by qRT-PCR. For each dilution analyzed, NucleoMag Pathogen provided superior assay sensitivity as compared to the MagMAX kit. Back