NucleoSnap Finisher Midi

Description

Eluates from anion exchange plasmid preparations such as NucleoBond midipreps and maxipreps contain high salt concentrations. Macherey-Nagel has developed a new vacuum-driven accessory, the NucleoSnap Finisher Midi/Maxi kits, that allow you to desalt and concentrate plasmid DNA in a few minutes without any loss of yield or quality. These kits are the fastest way to desalt and concentrate DNA after your NucleoBond plasmid DNA preps. NucleoSnap Finisher Midi/Maxi kits supersede the commonly used and time-consuming isopropanol precipitation and centrifugation steps by allowing rapid DNA precipitation and concentration of large volumes under vacuum. Cat. # 740434.10 includes sufficient quantities of reagents and materials for processing of 10 samples. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Required Products NucleoBond PC 20 20 20 preps for the isolation of plasmid DNA - NucleoBond AX 20 Columns, buffers, RNase A Back NucleoBond PC 20 100 100 preps for the isolation of plasmid DNA - NucleoBond AX 20 Columns, buffers, RNase A Back Back NucleoBond Xtra Midi 10 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Back Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF.Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells ODV200 vs. ODV600 using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount ODV400 . ODV was calculated by multiplying the optical density of each culture measured at 600 nm times the culture volume. Back Back Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi.Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit in each case, the maximum input volume specified by the corresponding kit protocol was processed . Yields of plasmid DNA were 60100 % higher with the NucleoBond kits than the Qiagen kits Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit . NucleoBond Xtra Midi 50 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF.Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells ODV200 vs. ODV600 using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount ODV400 . ODV was calculated by multiplying the optical density of each culture measured at 600 nm times the culture volume. Back Back Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi.Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit in each case, the maximum input volume specified by the corresponding kit protocol was processed . Yields of plasmid DNA were 60100 % higher with the NucleoBond kits than the Qiagen kits Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit . Back Back Back Use Midi prep and Midi prep Xtra Plus to purify plasmid DNA in 30 min without the use of vacuum or centrifugation. Typical yield >500 mug of ultra-pure plasmid DNA. Back Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF.Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells ODV200 vs. ODV600 using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount ODV400 . ODV was calculated by multiplying the optical density of each culture measured at 600 nm times the culture volume. Back Back Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi.Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit in each case, the maximum input volume specified by the corresponding kit protocol was processed . Yields of plasmid DNA were 60100 % higher with the NucleoBond kits than the Qiagen kits Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit . Back NucleoBond Xtra Midi EF 10 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF.Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells ODV200 vs. ODV600 using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount ODV400 . ODV was calculated by multiplying the optical density of each culture measured at 600 nm times the culture volume. Back Back Back Back NucleoBond Xtra Midi EF 50 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF.Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells ODV200 vs. ODV600 using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount ODV400 . ODV was calculated by multiplying the optical density of each culture measured at 600 nm times the culture volume. Back Back Back Back Back NucleoBond PC 100 20 20 preps for the isolation of plasmid DNA - NucleoBond AX 100 Columns, Folded Filters, buffers, RNase A Back Back Back NucleoBond PC 100 100 100 preps for the isolation of plasmid DNA - NucleoBond AX 100 Columns, NucleoBond Folded Filters, buffers, RNase A Back Back Back