NucleoSnap Plasmid Midi

Description

Endotoxins are copurified during plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Macherey-Nagel has developed a new column format and a novel buffer chemistry to enable vacuum-processed isolation of transfection-grade plasmid DNA in a midiprep format. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Back Back Back Higher, more consistent yields with NucleoSnap Plasmid Midi.Multiple plasmid preps were performed in parallel from the indicated volumes of bacterial cultures using NucleoSnap Plasmid Midi, the Plasmid Plus Midi Kit from Qiagen Q , and the ZymoPURE II Plasmid Midiprep Kit from Zymo Z , respectively. Quantification of purified plasmid from each prep indicated that NucleoSnap Plasmid Midi provided higher yields than the Qiagen kit and more consistent results than the Zymo kit. Required Products The NucleoVac 24 Vacuum Manifold allows the parallel processing of silica columns for nucleic acid purification by vacuum. The silica columns e.g., NucleoSpin and NucleoSnap columns can be attached to the upper Luer openings of the vacuum manifold. The binding and washing steps of the NucleoSpin or NucleoSnap procedures are carried out by vacuum instead of centrifugation, enabling time-saving procedures NucleoSpin products and processing of large sample volumes NucleoSnap products . Back Back Back