NucleoSpin eDNA Water

Description

The NucleoSpin eDNA Water kit enables rapid purification of environmental DNA eDNA from diverse water samples with reliable results and minimal risk of DNA contamination. The combination of silica matrix and silica membrane technologies allows for efficient isolation of high-quality DNA in about an hour, providing a convenient alternative to the overnight incubation steps typical of eDNA workflows. The XS columns provided with the kit are treated with ethylene oxide in order to minimize the risk of DNA contamination. NucleoSpin eDNA Water is compatible with a variety of filters and filtration systems, such as conventional bottle top filters e.g., 45 mm as well as cartridge filters e.g., Sterivex . Further, an alternative protocol for direct eDNA precipitation from unfiltered water is provided, enabling isolation of free DNA. These properties make NucleoSpin eDNA Water ideal for studies of freshwater and saltwater ecosystems with varying levels of organic and inorganic matter. Cat. # 740402.10 includes reagents and materials for processing 10 samples. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Consistent performance of NucleoSpin eDNA Water with diverse round filters.500-ml samples of creek water were filtered through the following different types of round filters, which were then processed using NucleoSpin eDNA Water: regenerated cellulose RC , cellulose nitrate NC , cellulose acetate CA , polyester PE , polycarbonate PC , mixed cellulose ester CM and paper. As demonstrated by the resulting Ct values obtained in a downstream qPCR analysis, performance of DNA isolation was similar for each of the filter types and comparable to the performance observed with the recommended ethylene oxide-treated glass fiber filter GF , which provides the best flow rate and lowest risk of clogging. Back Sample preparation workflow for NucleoSpin eDNA Water.Samples can be obtained either by filtration with round filters or filter cartridges e.g., Sterivex . DNA is liberated from the filter by a strong lysis buffer. Alternatively, eDNA can be precipitated from 40 ml of unfiltered water using Buffer PREC, Cat. # 740568 . NucleoTrap Suspension beads efficiently bind the DNA while minimizing carryover of contaminants. Following washing and drying steps, the bead suspension is transferred to a NucleoSpin XS column, from which pure eDNA is eluted. The purification steps take less than 70 min, while total preparation times vary depending on the sampling and/or filtration setup. Back Seasonal traceability of European toad in a freshwater pond.In Germany, European toads become active after hibernationusually in Marchand typically start spawning in April. The presence of toads and/or spawn and tadpoles can be monitored by isolation of eDNA and subsequent PCR analysis. The NucleoSpin eDNA Water kit was used in combination with NucleoSpin Filter Midi to isolate DNA from a freshwater pond at the indicated timepoints, and PCR was performed to detect toad-specific or general eukaryotic DNA using corresponding primer pairs. Back Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits.eDNA was purified from running water creek and static water pond using the NucleoSpin eDNA Water kit in parallel with two competitor kits BT and PW and analyzed by qPCR for metazoan DNA. As demonstrated by the lower Ct values, NucleoSpin eDNA Water provided higher sensitivity than the competitors. Back Compatibility of NucleoSpin eDNA Water with different water filtration systems.Samples of creek water were filtered through a glass fiber filter GF as well as several filtration systems commonly used in conjunction with eDNA purification. The NucleoSpin eDNA Water kit was then used to purify eDNA from each filter unit, and eluates were analyzed by qPCR for metazoan DNA. Purification from each filtration system yielded comparable results, as demonstrated by the consistent Ct values. Back