NucleoSpin Gel and PCR Clean-Up

Description

NucleoSpin Gel and PCR Clean-up 50 50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products.The kit includes one buffer for both applications. The purification procedure from enzymatic reactions e.g., PCR allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE 5 mM Tris/HCl, pH 8.5 . Back