NucleoSpin RNA Clean-up Maxi

Description

The NucleoSpin RNA Clean-up Maxi kit enables cleanup of large amounts of RNA from crude preparations or enzymatic reactions using a simple maxi spin column-based procedure. The kit allows for processing of up to 35 mg of crude RNA, with typical recovery rates ranging from 8595 %. To prevent RNA degradation, samples are mixed with a concentrated solution of chaotropic ions and ethanol, resulting in immediate RNase inactivation. Following adsorption to the silica membrane, RNA is washed three times with two different wash buffers to remove impurities, and eluted under low ionic strength conditions with RNase-free water. With the NucleoSpin RNA Clean-up Maxi kit, 6 samples can be processed in 30 minutes. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Efficient RNA recovery from varying inputs.The NucleoSpin RNA Clean-up Maxi kit was used to process three different crude RNA eluates containing 6.835.4 mg of RNA. Following cleanup according to the standard protocol, 6.630.1 mg of RNA was recovered, corresponding to recovery rates of 8596 %.