NucleoSpin RNA Stool

Description

The NucleoSpin RNA Stool kit is designed for efficient isolation of RNA, including small RNA species, from fresh and frozen stool samples. The kit employs silica-membrane technology in a mini spin column format, and typically yields 1030 mug of RNA from 200-mg inputs of fresh or frozen stool. The kit workflow allows for processing of 10 samples in parallel in approximately 70 min. Cat. # 740130.10 includes sufficient quantities of reagents and materials for processing of 10 samples. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back NucleoSpin RNA Stool procedure. Back Highest yield with NucleoSpin RNA Stool.Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool MN , whereas 250-mg samples were processed with the competitor kits. For protocol Q, the optional phenol-based lysis step was included. For each sample and protocol, extractions were performed in triplicate. For each sample, NucleoSpin RNA Stool provided 2060 % higher yields compared to the competitor kits. Back Highest RNA purity and integrity with NucleoSpin RNA Stool.Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool MN , whereas 250-mg samples were processed with the competitor kits. For protocol Q, the optional phenol-based lysis step was included. For each sample and protocol, four extractions were performed. For each sample, NucleoSpin RNA Stool yielded RNA of the highest purity A260/A280, top and integrity RIN, bottom compared to the competitor kits. Back NucleoSpin RNA Stool shows best performance in qPCR.Two human stool samples were processed within 4 hours after collection using standard protocols including an on-column DNase digestion step. As indicated in the corresponding protocols, 200-mg stool samples were processed using NucleoSpin RNA Stool MN , whereas 250-mg samples were processed with the competitor kits. For protocol Q, the optional phenol-based lysis step was included. For samples processed with NucleoSpin RNA Stool and protocol Q, RNA was eluted in 100-mul volumes, whereas eluates from protocol Z were each adjusted to a final volume of 100 mul to obtain comparable RNA concentrations for each protocol. For each sample and protocol, extractions were performed in triplicate. For each sample, RNA obtained with NucleoSpin RNA Stool provided better performance in qPCR compared to RNA prepared with the competitor kits.