NucleoSpin totalRNA FFPE

Description

The NucleoSpin totalRNA FFPE kit 50 preps allows for the purification of small and large RNA species from standard FFPE samples <50 mg tissue into a 3050 mul elution volume. The kit includes NucleoSpin RNA Columns, Collection Tubes, Paraffin Dissolver, buffers, Proteinase K, and RNase-free rDNase. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back NucleoSpin totalRNA FFPE provides better RT-PCR performance lower CTvalues than competitor kits, indicating higher RNA yields.Large e.g., mRNA and small e.g., miRNA RNA were isolated from 4 x 10 mum FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits Q, A, P . Quantification of mRNA and miRNA was performed using qRT-PCR. Low CTvalues indicate high RNA yields.The amplification target consists of a 230-base fragment of the beta2-microglobulin gene.miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems. Back NucleoSpin totalRNA FFPE provides more efficient gDNA removal than competitor kits.Large e.g., mRNA and small e.g., miRNA RNA were isolated from 4 x 10 mum FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and three competitor kits Q, A, P . Residual DNA was assayed by amplifying a 191-bp fragment of the mGAPDH gene. Higher CTvalues correspond to lower amounts of residual DNA. Back The higher concentration of total RNA obtained from XS columns allows efficient, sensitive qRT-PCR from very small FFPE samples.Trace amounts of FFPE samples 1 x 15 mum mouse spleen and 1 x 10 mum mouse heart were used to isolate mRNA and miRNA with NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS. The RNA was eluted in 50 mul using a NucleoSpin totalRNA FFPE mini spin column and 20 mul using a NucleoSpin totalRNA FFPE XS column . The mRNA and miRNA eluted from the XS column at higher concentrations, reducing the CTvalue by at least one cycle. When using standard FFPE sample amounts, NucleoSpin totalRNA FFPE which uses mini spin columns is recommended in order to avoid possible column overloading.The amplification target consists of a 230-base fragment of the beta2-microglobulin gene.miRNA was quantified using the miR-16 MicroRNA Assay in the TaqMan MicroRNA Reverse Transcription Kit from Applied Biosystems Back Back