NUDT1 CRISPR Knockout CHP-134 Cell Line - Clone 4

Description

CRISPR-Cas9 genome editing was employed to generate knockout clones of NUDT1 in CHP-134 neuroblastoma cells. Cells were transfected with a Cas9 and guide RNA expression vector pSpCas9n BB -2A-GFP , in which the original GFP-targeting sequence was replaced with sgRNAs targeting NUDT1. To facilitate targeted disruption of the open reading frame, a corresponding homology-directed repair HDR template containing 5 and 3 homology arms flanking a puromycin resistance cassette was cloned into the pJET1.2/blunt vector. Following transfection and puromycin selection, resistant clones were clonally expanded.