NUTRIENT AGAR (WITH 3% SALT)

Description

Vibrios are fairly easy to isolate from both clinical and environmental materials, though some species may require growth factors and vitamins. Vibrio parahaemolyticus is the leading cause of bacterial diarrhea associated with the consumption of contaminated food products. Media can be made selective for Vibrios by adding appropriate selective agents. High concentrations of NaCl and alkaline pH have also been used to select certain Vibrio species, based on their ability to grow at pH values above 8.0 and at 3% or higher concentrations of NaCl. Vibrio cholerae is a non-halophilic Vibrio, which cannot grow in media with a concentration of sodium chloride greater than 5-6% and is able to grow in media lacking NaCl (1). High Salt Nutrient Agar is recommended for the isolation, cultivation and confirmation of salt-tolerant Vibrio species in products intended for human consumption or animal feeding stuffs in accordance with ISO Committee under specification ISO/DIS 8914:1990.