One Step PrimeScriptTM III RT-qPCR Mix, with UNG

Description

One Step PrimeScript III RT-qPCR Mix, with UNG is a dedicated reagent for one-step, real-time, probe-based RT-qPCR using the 5 nuclease method . This 2X premix does not freeze at its storage temperature of -20C, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube. Because the premix contains Uracil N-Glycosylase UNG , which degrades amplification products from the previous reaction, it can prevent carryover contamination even when a specific target is repeatedly tested for the purpose of inspection, etc. The reverse transcription reaction uses the novel PrimeScript III RTase, which displays increased heat tolerance up to 55C while maintaining the specificity and extensibility of PrimeScript RTase. This allows cDNA synthesis from RNA with a more complex secondary structure. After cDNA synthesis, TaKaRa Taq HS performs highly specific and efficient PCR amplification, while the fluorescence emitted by the probe is detected in real-time. One Step PrimeScript III RT-qPCR Mix, with UNG is also highly resistant to a wide variety of inhibitory substances such as heparin blood and humic acid soil , allowing stable one-step real-time RT-qPCR to be performed on a wide range of samples. This product can be used for various applications such as gene expression, RNA virus detection, etc. Cat. # RR601A contains enough reagents to perform 200 25-mul reactions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back