OneScript Hot cDNA Synthesis Kit

Description

OneScript Hot cDNA Synthesis Kit is the first kit in the world that allows for efficient and robust cDNA synthesis at 60oC. All other cDNA synthesis kits on the market are optimized for the reaction to be performed at 42-55oC, which is sufficient for most RNA template, but fails on more difficult templates. abm is the only company in the world to have a reverse transcriptase engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples due to its incredible thermostability at 60-72oC. The enzyme is a mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase M-MLV RT that efficiently reverse transcribes low-abundance or partially degraded RNA and exhibits enhanced resistance to common inhibitors such as reagents used during RNA extraction and contaminants from biological samples. It features genetic modifications that abolish RNase H activity to achieve thermal stability, allowing the RNA template to remain intact during first-strand synthesis for higher cDNA yield and fidelity. Its high processivity and sensitivity enable rapid synthesis of full-length cDNA fragments in a fraction of the time required by leading competitors. OneScript Hot is formulated with abms RNaseOFF Ribonuclease Inhibitor, which provides improved resistance to oxidation compared to traditional, oxidation-sensitive human RNase inhibitors. RNaseOFF remains stable even under very low concentrations of DTT <1 mM , ensuring superior RNA protection and reliability in demanding experimental conditions. Product Features: Extremely thermostable cDNA synthesis at 60-72oC Transcribes degraded samples and resists inhibitors/contaminants High cDNA yields from difficult or ultra-low RNA samples Included in abms PCR Buffet Program Product Component Quantity OneScript Hot Reverse Transcriptase 100 rxn 100 mul 5X RT Buffer 400 mul Oligo dT 10 muM 100 mul Random Primers 10 muM 100 mul dNTP 10 mM 100 mul Nuclease-Free H2O 2 x 1.0 ml ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization . This product is equivalent to RScript Hot CDNA Synthesis Kit Cat. No. G594EU .