pAcGFP1 Vector

Description

pAcGFP1 encodes a monomeric green fluorescent protein fromAequorea coerulescens. pAcGFP1 is a prokaryotic expression vector that also serves as a convenient source of fluorescent protein cDNA. In this vector, the full-length human-codon-optimized cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Basic fluorescent protein vector map.Use this bacterial expression vector as a source of the fluorescent protein gene.Note: There is a stop codon at the 5 end of the 3 MCS. The 3 MCS should not be used for cloning. Back AcGFP1 is a monomeric protein.Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance A280 and chromophore excitation A477 of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa.Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient.Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins 7.5 mug were separated by SDS-PAGE electrophoresis 12 % acrylamide . In both the boiled denatured and unboiled nondenatured samples, AcGFP1 runs as a uniform band of 30 kDa due to its monomeric structure. The unboiled nondenatured DsRed-Express runs at a much higher molecular weight than its denatured boiled counterpart due to its oligomeric structure. Back