pDsRed-Express Vector

Description

pDsRed-Express is a prokaryotic expression vector that encodes DsRed-Express, a variant ofDiscosomasp. red fluorescent protein DsRed . pDsRed-Express is primarily intended to serve as a source of DsRed-Express cDNA. The flanking MCS regions make it possible to excise the DsRed-Express coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce DsRed-Express protein. DsRed-Express contains nine amino acid substitutions which improve the solubility of the protein, reduce the time from transfection to detection of red fluorescence, and decrease the level of residual green emission. When DsRed-Express is expressed in mammalian cell cultures, red-emitting cells can be detected by either fluorescence microscopy or flow cytometry 812 hours after transfection. Although DsRed-Express most likely forms the same tetrameric structure as wild-type DsRed, DsRed-Express displays a reduced tendency to aggregate. The DsRed-Express coding sequence is human codon-optimized for high expression in mammalian cells. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Basic fluorescent protein vector map.Use this bacterial expression vector as a source of the fluorescent protein gene.Note: There is a stop codon at the 5 end of the 3 MCS. The 3 MCS should not be used for cloning. Back