pDsRed-Monomer Vector

Description

pDsRed-Monomer encodes DsRed-Monomer, a monomeric mutant of theDiscosomasp. red fluorescent protein DsRed. In this vector, the DsRed-Monomer coding sequence is flanked by separate MCS regions, making it possible to excise and shuttle the DsRed-Monomer coding region into other vector systems. pDsRed-Monomer is primarily intended to serve as a source of DsRed-Monomer cDNA. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Basic fluorescent protein vector map.Use this bacterial expression vector as a source of the fluorescent protein gene.Note: There is a stop codon at the 5 end of the 3 MCS. The 3 MCS should not be used for cloning. Back Back DsRed-Monomer is a monomeric protein.Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins 100 mug were analyzed by FPLC gel filtration chromatography. Overall absorbance A280 and chromophore excitation A557 of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time 39 min corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time 33 min corresponding to a molecular weight of 89 kDa.Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins 7.5 mug were separated by SDS PAGE electrophoresis 12 % acrylamide . In both the boiled denatured and unboiled nondenatured samples, DsRed-Monomer fluorescent protein runs as a uniform band of 30 kDa due to its monomeric structure. The unboiled nondenatured DsRed-Express runs at a much higher molecular weight than its boiled denatured counterpart due to its tetrameric structure.