pDsRed2-Bid Vector

Description

pDsRed2-Bid encodes a fusion ofDiscosomasp. red fluorescent protein DsRed2 and Bid, a member of the Bcl-2 family. In healthy, non-apoptotic cells, Bid resides in the cytosol as soluble protein. Upon induction of apoptosis, Bid translocates to mitochondria. In cells expressing the Bid-DsRed2 fusion, the translocation can be detected by fluorescence microscopy. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Monitoring Bid activation with the pDsRed2-Bid Vector.3T3 cells were transiently transfected with pDsRed2-Bid Panels A&B . Approximately 24 hr later, cells were incubated with 700 nM staurosporine, an apoptosis inducing agent, at 37C for 3 hr. Cells were then fixed and examined with a Zeiss Axioskop equipped with the appropriate light filters. In untreated cells Panel A , the Bid reporter Bid-DsRed2 distributes uniformly in the cytosol, as expected. Following induction Panel B , the reporter translocates to the surface of mitochondria, forming intensely fluorescent clustersclear evidence that the Bid pathway has been activated.