pDsRed2-ER Vector

Description

pDsRed2-ER is a mammalian expression vector designed to label the endoplasmic reticulum in living cells. The vector encodes a fusion consisting ofDiscosomasp. red fluorescent protein DsRed2 ; the endoplasmic reticulum ER targeting sequence of calreticulin, fused to the 5 end of DsRed2; and the ER retention sequence, KDEL, fused to the 3 end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation. pDsRed2-ER can be introduced into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back AcGFP1 is ideal for multicolor and fluorescence microscopy applications.AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy.Panel A.pAcGFP1-Mito mitochondria and pDsRed2-Nuc nucleus in HEK 293 cells.Panel B.pAcGFP1-Golgi Golgi apparatus and pDsRed2-Nuc nucleus in HEK 293 cells. Back Organelles targeted by the subcellular localization vectors. Back Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure.The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants. Back