pEF1alpha-DsRed-Express2 Vector

Description

pEF1alpha-DsRed-Express2 is a mammalian expression vector that constitutively expresses the red fluorescent protein DsRed-Express2, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of DsRed-Express2 is driven by the human elongation factor 1 alpha EF1alpha promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Robust expression ofDsRed-Expressand DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells.Mononuclear bone marrow cells were transduced with retroviral vectors encoding DsRed-Express Panel A , DsRed-Express2 Panel B , or EGFP Panel C , and fluorescent cells were sorted 87 hr later. Red and green fluorescence signals were detected using the PE and FITC filter sets, respectively. The lines represent gates defined by analyzing untransduced cells. Back Map schematic of the plasmid choices that are available carrying the EF1-alpha promoter.IRES: internal ribosome entry sequence; FP1: fluorescent protein AcGFP1, DsRed-Monomer, or mCherry ; FP2: fluorescent protein mCherry or ZsGreen1 ; MCS: multiple cloning site. Back