pET Express & Purify KitHis60 (In-Fusion Ready)

Description

The pET Express & Purify Kit-His60 In-Fusion Ready allows you to use In-Fusion cloning technology to express a protein of interest with an N- or C-terminal 6xHN tag inE. coli. The system contains IPTG-inducible, pET-based bacterial expression vectors that have been prelinearized to allow In-Fusion cloning via the included In-Fusion HD Enzyme Premix. These vectors contain a T7/lacpromoter and polyhistidine tag sequence for high-level expression of his-tagged proteins, which can be easily purified with the high-capacity His60 Ni Gravity Columns included in the kit. The kit also contains a control vector that expresses an N-terminal, 6xHN-tagged, GFPuv fusion protein. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Back Back Back