pGFPuv Vector

Description

pGFPuv encodes a variant ofAequorea victoriagreen fluorescent protein GFP that has been optimized for brighter fluorescence when excited by standard UV light. The GFPuv coding sequence is flanked by separate MCSes at the 5 and 3 ends, so the GFPuv gene can be excised from pGFPuv. Alternatively, the GFPuv coding sequences can be amplified by PCR. pGFPuv carries the cycle 3 variant of GFP. This gene was cloned between the two MCSs of the pUC19 derivative pPD16.43. The GFPuv gene can be easily excised from pGFPuv. Alternatively, the GFPuv coding sequence can be amplified by PCR. The GFPuv gene was inserted in frame with thelacZinitiation codon from pUC19 so that a beta-galactosidase-GFPuv fusion protein is expressed from thelacpromoter inE. coli.Note, however, that if you excise the GFPuv coding sequence using a restriction site in the 5 MCS, the resulting fragment will encode the native i.e., non-fusion GFPuv protein. The pUC backbone of pGFPuv provides a high copy number origin of replication and ampicillin resistance gene for propagation inE. coli. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back pGFP and pGFP-UV vector maps.Note: There is a stop codon at the 5 end of the 3 MCS. The 3 MCS should not be used for cloning.