Phalloidin-iFluor 555 Reagent

Description

How the assay works Phalloidin-iFluor 555 Reagent equivalent to Alexa Fluor 555-labeled phalloidin selectively binds to F-actins.It can be detected with a fluorescent microscope at Ex/Em = 556/574 nm. Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized. Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step. When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation. Phalloidin-iFluor 555 Reagent Protocol Summary - Prepare samples in microplate wells - Remove liquid from samples in the plate - Add Phalloidin-iFluor 555 100 muL/well - Stain the cells at room temperature for 20 to 90 minutes - Wash the cells - Examine the specimen under microscope with TRITC filter How other researchers are using Phalloidin-iFluor 555 Reagent has been used in a variety of sample type including: - Rabbit corneas 1 - Human Mesodermal Progenitor Cells 2 - Humanumbilical vein endothelial cell line 3 References: 1 - Song E et al. 2024; 2 - Stberl N et al. 2023; 3 - Songjang W et al. 2023. Related and recommended products Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 ab176753 , Phalloidin-iFluor 647 ab176759 and Rhodamine Phalloidin ab235138 , or read the phalloidin staining protocol.