pHcRed1-Nuc Vector

Description

pHcRed1-Nuc Vector encodes a far-red fluorescent protein HcRed1 fused with three copies of the nuclear localization signal NLS of the SV40 T-antigen. The NLS sequences are fused to the 3-end of HcRed1. HcRed1 was generated by mutagenesis of a non-fluorescent chromoprotein from the reef coralHeteractis crispa. The coding sequence for HcRed1 is human codon-optimized for higher expression in mammalian cells. pHcRed1-Nuc is designed for visualizing the nucleus in living or fixed cells by fluorescence microscopy. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back AcGFP1 is ideal for multicolor and fluorescence microscopy applications.AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy.Panel A.pAcGFP1-Mito mitochondria and pDsRed2-Nuc nucleus in HEK 293 cells.Panel B.pAcGFP1-Golgi Golgi apparatus and pDsRed2-Nuc nucleus in HEK 293 cells. Back Organelles targeted by the subcellular localization vectors. Back Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure.The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.