pHcRed1 Vector

Description

pHcRed1 is a bacterial expression vector which encodes the far-red fluorescent protein, HcRed1. HcRed1 was generated by mutagenesis of a non-fluorescent chromoprotein from the reef coralHeteractis crispa. The HcRed1 coding sequence has been human codon-optimized for higher expression in mammalian cells. The sequence upstream of HcRed1 has been converted to a Kozak consensus sequence to further increase the translation efficiency in eukaryotic cells. The HcRed1 coding sequence is flanked by distinct multiple cloning sites MCS so that the gene can be easily excised from pHcRed1. Alternatively, the HcRed1 coding sequence can be amplified by PCR. InE. coli,HcRed1 is expressed from thelacpromoter as a fusion with several additional amino acids, including the first five amino acids of theLacZprotein. Note, however, that if you excise the HcRed1 coding sequence using a restriction site in the 5 MCS, the resulting fragment will encode the native i.e., non-fusion HcRed1 protein. pHcRed1 is a pUC 19 derivative pPD16.43 which provides a high-copy-number origin of replication as well as an ampicillin resistance gene for propagation and selection inE.coli. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1. Back Basic fluorescent protein vector map.Use this bacterial expression vector as a source of the fluorescent protein gene.Note: There is a stop codon at the 5 end of the 3 MCS. The 3 MCS should not be used for cloning.