Phosphatase Inhibitor Cocktail II (50×)

Description

During cell and tissue lysis, large amounts of endogenous protein phosphatases are released. These enzymes catalyze the dephosphorylation of phosphorylated proteins, leading to abnormal protein phosphorylation status that deviates from the normal physiological level. Therefore, when performing experiments involving phosphorylated protein detection and protein kinase activity assays after sample lysis, such as Western blotting and co-immunoprecipitation, exogenous protein phosphatase inhibitors must be added to effectively block protein dephosphorylation and maintain the inherent phosphorylation state of proteins in samples.