Phospho-ERK 8 (Thr175/Y177) Rabbit Polyclonal Antibody

Description

EnkiLife offers the Phospho-ERK 8 (Thr175/Y177) Rabbit Polyclonal Antibody. This antibody reacts with Human,Mouse species and is suitable for WB,IHC,ICC/IF,ELISA application. Alternative Names of Immunogen: MAPK15; ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8. The product boasts remarkable high specificity, outstanding reproducibility, and exceptional affinity, making it a reliable and superior choice for your needs. Dilution Ratio: WB 1:500-1:2000,IHC 1:100-1:300,ICC/IF 1:50-1:200,ELISA 1:5000-1:10000 Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein. domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation. domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1. function:In vitro, phosphorylates MBP. PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase. similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. similarity:Contains 1 protein kinase domain. subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1. tissue specificity:Widely expressed with a maximal expression in lung and kidney.