Phospho-MSK1 (Ser212) Rabbit Polyclonal Antibody

Description

EnkiLife offers the Phospho-MSK1 (Ser212) Rabbit Polyclonal Antibody. This antibody reacts with Human,Mouse species and is suitable for WB,IHC,ICC/IF,ELISA application. Alternative Names of Immunogen: RPS6KA5; MSK1; Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL. The product boasts remarkable high specificity, outstanding reproducibility, and exceptional affinity, making it a reliable and superior choice for your needs. Dilution Ratio: WB 1:500-1:2000,IHC 1:100-1:300,ICC/IF 1:50-1:200,ELISA 1:20000-1:40000 Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein. cofactor:Magnesium. enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process. function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). miscellaneous:Enzyme activity requires the presence of both kinase domains. PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. similarity:Contains 1 AGC-kinase C-terminal domain. similarity:Contains 2 protein kinase domains. subcellular location:Predominantly nuclear. Partially cytoplasmic. subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA. tissue specificity:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. Research Area:Insulin Receptor; Regulates Angiogenesis; MAPK_ERK_Growth;MAPK_G_Protein; B Cell Receptor; AMPK