plRES2-ZsGreen1 Vector

Description

pIRES2-ZsGreen1 is a bicistronic expression vector containing ZsGreen1 as a marker for transfection efficiency. It encodes a human-codon-optimized variant of theZoanthussp. green fluorescent protein, ZsGreen, which has been engineered for brighter fluorescence and higher expression in mammalian cells. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus EMCV internal ribosomal entry site IRES . The IRES sequence allows the gene of interest and ZsGreen1 to be translated simultaneously from the same mRNA transcript. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Use of AcGFP1 for fusions and fluorescence microscopy applications.Panels AandB. Activation of Protein Kinase C alpha was monitored with Living Colors AcGFP1.Panel A. HEK 293 cells were stably transfected with a plasmid encoding AcGFP1 fused to PKC alpha.Panel B. Cells were induced with 1.5 mug/ml PMA for 3 min. The PKC alpha-AcGFP1 fusion moves from the cytosol to the plasma membrane, a result consistent with the known mobilization pattern of PKC alpha.Panel C. HeLa cells were transiently transfected with pAcGFP1-Actin and visualized by fluorescence microscopy. Back Back Back