pLVX-AcGFP1-N1 Vector

Description

The pLVX-AcGFP1-N1 vector is an HIV-1-based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a variant of the green fluorescent protein GFP fromAequoreacoerulescens. pLVX-AcGFP1-N1 allows cloning of a cDNA into the multiple cloning site MCS upstream of the coding sequence for AcGFP1. The cDNA is subsequently expressed as a fusion to the N-terminus of the AcGFP1 protein. Thus, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Lentiviral vectors with fluorescent proteins.Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal , Rev response element RRE , and central polypurine tract/central termination sequence cPPT/CTS from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element WPRE . Vectors can express your protein fused at its N- or C- terminus to either a green AcGFP1 or red DsRed-Monomer fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 shown , mCherry, or tdTomato. Back