pLVX-DsRed-Express2-C1 Vector

Description

This lentiviral expression vector encodes aDsRed-Express2fluorescent protein tag. The protein is a rapidly maturing tetrameric fluorescent protein fromDiscosoma sp.that has minimal cytotoxicity, high solubility, and exhibits strong and stable expression in mammalian cells. Inserting a cDNA in the MCS downstream of the DsRed-Express2 coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using the Lenti-X Packaging Single Shots VSV-G and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Lentiviral vectors with fluorescent proteins.Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal , Rev response element RRE , and central polypurine tract/central termination sequence cPPT/CTS from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element WPRE . Vectors can express your protein fused at its N- or C- terminus to either a green AcGFP1 or red DsRed-Monomer fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 shown , mCherry, or tdTomato. Back Robust expression ofDsRed-Expressand DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells.Mononuclear bone marrow cells were transduced with retroviral vectors encoding DsRed-Express Panel A , DsRed-Express2 Panel B , or EGFP Panel C , and fluorescent cells were sorted 87 hr later. Red and green fluorescence signals were detected using the PE and FITC filter sets, respectively. The lines represent gates defined by analyzing untransduced cells.