POLYMYXIN B SELECTIVE SUPPLEMENT

Description

Rehydrate the contents of one vial aseptically with 2 ml sterile distilled water. Mix well and aseptically add it to 475 ml of sterile, molten TM 1120 – BACILLUS CEREUS AGAR BASE or to 450 ml of TM 1207 – KG AGAR BASE / TM 1881 – BACILLUS CEREUS SELECTIVE AGAR BASE (MYP) (ISO 7932:2004) / TM 197 – MYP AGAR BASE (PHENOL RED EGG YOLK POLYMYXIN AGAR BASE) (IS : 5887 (Part VI) 1976, reaffirmed 2005) / TMV 197 – MYP AGAR BASE (PHENOL RED EGG YOLK POLYMYXIN AGAR BASE) (VEG.) / TM 1843 – MANNITOL EGG YOLK POLYMYXIN AGAR (ISO 7932:2004, ISO 21871:2006) along with 25 ml/50 ml Egg Yolk Emulsion to make a total volume of 500 ml or to 500 ml of TM 281 – SDS AGAR (SODIUM DODECYL SULPHATE POLYMYXIN SUCROSE AGAR) (as per APHA) / TM 851 – SALT POLYMYXIN BROTH BASE / TM 1848 – SALT POLYMYXIN BROTH BASE (ISO 8914:1990) / TM 1931 – SOYBEAN CASEIN DIGEST MEDIUM BASE W/O POLYMYXIN / TM 1523 – CHROMOGENIC BACILLUS AGAR. Mix well and pour into sterile petri plates / tubes.